Deborah Littlejohn has been a Design Fellow at the University of Minnesota Design Institute since February 2002 after co-designing the Midtown Crossings Knowledge Map. She designs and manages a variety of DI projects, including its graphic design, promotional materials, and publications. Most recently, she designed and edited Metro Letters: A Typeface for the Twin Cities, a project with the DI's Twin Cities Design Celebration 2003.

With her partner Santiago Piedrafita, Deborah runs TWO, a studio that produces print and digital design for a variety of clients, mainly in the cultural sector.

Littlejohn's work has been exhibited nationally and internationally, and has been featured in numerous publications including I.D., Typography Now Two, Digital Type, Metropolis, Graphis, Communication Arts, and Affiche. She has received awards from the American Center for Design, the American Institute of Graphic Arts, the American Association of Museums, I.D. Magazine, among others. She was co-curator and editor of the digital design and interactive media exhibition and publication New Genre Hybrid Language.

Littlejohn taught interactive media, motion graphics and graphic design for several years at the Minneapolis College of Art and Design. She has been a visiting lecturer at California Institute of the Arts, Cranbrook Academy of Art, North Carolina State University and Rhode Island School of Design. She received her MFA from CalArts in 1994.