Dynamics Imaging of Biomolecular Assemblies

 

Outreach

(1) Heikal Group in the News:

 

(2) Science Exhibit for Kids and Science Fair Judging:

  • "Project Judge" at the NE MN Regional Science Fair (February 5th, 2011), University of Minnesota Duluth, Duluth, MN.
  • "Project Judge" at the Lakeview Christian Academy Science Fair (January 17th, 2011), Lakeview Christian Academy, Duluth, MN.
  • "Project Judge" at the State Science and Engineering Fair (March 27, 2010), Minnesota Academy of Science, Crowne Plaza Hotel, St. Paul, MN.
  • Explore Science” booth for kids and their family, the Annual Crawford County (PA) Fair, Meadville, PA (2005, 2006, 2007 and 2008). This was in collaboration with 4-H, and Dr. Erin Sheets (College of Pharmacy, University of Minnesota). This science exhibit was featured in Erie Times-News, Page 7A, Wednesday, August 20, 2008.

 

(3) Selected Invited Talks:

Heikal, Ahmed A. "Bioenergetics and Diffusion in the Crowded Milieu of Living Cells". The 11th Annual Advanced Imaging Techniques Workshop, University of California-Berkeley, Berkeley, CA, January 29 - 31, 2014.

Heikal, Ahmed A. "Biomimetic Crowding Effects on the Reaction Kinetics of NADH-Enzyme Binding". The 10th Annual Advanced Imaging Techniques Workshop, University of California-Berkeley, Berkeley, CA, January 30 - February 01, 2013.

Heikal, A.A. "Single-molecule studies of Bodipy-FTY720, an immune suppressor, in cultured C3H10T1/2 cells". The 9th Annual Advanced Imaging Techniques Workshop, University of California-Berkeley, Berkeley, CA, January 18-20, 2012. [Sponsored by Molecular Imaging Center / Cancer Research Laboratory at UC Berkeley, Becker & Hickl GmbH (Berlin, Germany), and Boston Electronics.]

Heikal, A.A. "Associated anisotropy of cellular NAD(P)H for quantitative biochemistry in living cells: Experiment versus simulation". The 5th Fall Workshop on Advanced TCSPC techniques in Biomedical Sciences, Bethesda, MD, November 10-11, 2011. [Sponsored by Becker & Hickl GmbH (Berlin, Germany), Boston Electronics, and the National Institute of Health.]

Heikal, A.A. "Specialized lipid domains and IgE receptor signaling". Department of Physics, University of Minnesota, Minneapolis, MN, October 25, 2011.

Heikal, A.A. "Snapshots of specialized lipid domains in model and functional biomembranes". Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE, March 30, 2011.

Heikal, A.A. "Specialized lipid domains: From model systems to functional biomembranes". Department of Chemistry, Gustavus Adolphus College, St. Peter, MN, March 11, 2011.

Heikal, A.A. "Cellular Autofluorescence: Opportunities and Challenges". 8th Advanced Imaging Methods, University of California Berkeley, Berkeley, CA, January 19 - 21, 2011.

Heikal, A.A. "Multiparameteric micro-spectroscopy of genetically GFP-encoded MHC I during antigen processing in fibroblast cells". 4rd Boston Workshop on Advanced TCSPC Techniques, Boston, MA, October 11 - 12, 2010.

Heikal, A.A. Intracellular coenzymes as natural biomarkers for metabolic and mitochondrial activities, Advanced Imaging Methods in Cell Biology and Neuroscience Symposium, Creighton University, Omaha, Nebraska, April 30, 2010.

Heikal, A.A.Single-molecule studies of MHC class I proteins in fibroblast cells, Symposium: 5th Making Single Molecule Fluorescence Lifetime Measurements Simple, Brookhaven National Laboratory, April 26-28, 2010.

Heikal, A.A. Fluorescence dynamics perspective of MHC class I proteins in fibroblast cells, Department of Chemistry, University of North Dakota, Grand Forks, ND, February 05, 2010.

Heikal, A.A. Fluorescence dynamics imaging of MHC class I proteins in mouse fibroblast cells, 7th Advanced Imaging Methods, Berkeley, CA, January 20-22, 2010.

Heikal, A.A. Structural heterogeneity of GFP-tagged, major histocompatibility complex class I protein in mouse fibroblast cells. 3rd Boston Workshop on Advanced TCSPC Techniques, Boston, MA, October 15 - 17, 2009.

Heikal, A.A. Cellular Autofluorescence: Towards quantitative biochemistry of energy metabolism and redox state of living cells. Biophysics Colloquium, Cornell University, Ithaca, NY, November 5, 2008.

Heikal, A.A. Molecular dynamics perspective of membrane domains: from model systems to IgE receptor signaling in RBL mast cells. Department of Chemistry, University of Pennsylvania, Philadelphia, PA, October 16, 2008.

Heikal, A.A. Cellular autofluorescence: towards quantitative biochemistry of energy metabolism and redox state of living cells. 2nd Boston Workshop on Advanced TCSPC Techniques, Boston, MA, September 22-23, 2008.

Heikal, A.A. Fluorescence dynamics Imaging of lipid domains in synthetic and natural membranes. Department of Chemistry, Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Urbana, IL, September 3, 2008.

Heikal, A.A. Fluorescence dynamics imaging of biomembranes: IgE receptor signaling in RBL cells. Department of Chemistry and Biochemistry, Queens College, CUNY, Flushing, NY, May 5, 2008.

Heikal, A.A. Fluorescence dynamics imaging of biomembranes: IgE receptor signaling in RBL cells. Department of Chemistry, Michigan State University, East Lansing, MI, March 27, 2008.

Heikal, A.A. Fluorescence dynamics imaging of biomembranes: IgE receptor signaling in RBL cells. Department of Molecular Physiology & Biological Physics, University of Virginia, Charlottesville, VA, March 24, 2008.

Heikal, A.A. Intermolecular interactions in biomembranes: from engineered mimics to living cells. Center of Optical Technology, Lehigh University, Bethlehem, PA, October 8, 2007.

Heikal, A.A. Short-range dynamics imaging of lipid phases in synthetic and natural biomembranes. The American Chemical Society National Meeting, San Francisco, CA, September 11, 2006.

Heikal, A.A. Navigating cellular biocomplexity with molecular sensitivity: energy metabolism. Center of Optical Technology, Lehigh University, Bethlehem, PA, September 28, 2006.     

Heikal, A.A. Quantitative analysis of energy metabolism using intrinsic coenzymes. Department of Physics, University of Maine, Orono, ME, November 11, 2005.      

Heikal, A.A. Nanoscale functional imaging of mitochondria in living cells. Department of Biology, Pennsylvania State University, University Park, PA, April 26, 2005.      

Heikal, A.A. Monitoring metabolic activities in living cells using native biomolecules. Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA, April 4, 2005.

Heikal, A.A. Fluorescence characterization of internally labeled DNA with fluorescently tagged nucleotides. Biocomplexity IV: Regenerative Biology and Medicine, Indiana University, Bloomington, IN, May 17, 2003.

Heikal, A.A. Multiphoton fluorescence snapshots of energy metabolism and protein dynamics. Department of Physics and Astronomy, University of Pennsylvania, Philadelphia, PA, February 20, 2003.

Heikal, A.A. Multiphoton fluorescence snapshots of energy metabolism & protein dynamics. Department of Physics, Vanderbilt University, Nashville, TN, February 11, 2003.

Heikal, A.A. Multiphoton fluorescence snapshots of energy metabolism and protein dynamics. Biomedical Group, University of Massachusetts Medical School, Worcester, MA, January 30, 2003.

Heikal, A.A. Multiphoton fluorescence snapshots of energy metabolism and protein dynamics. Department of Physics, Indiana University, Bloomington, IN, December 2, 2002.

Heikal, A.A. Multiphoton fluorescence snapshots of energy metabolism and protein dynamics. Department of Physics, The College of Optics and Photonics (CREOL), University of Central Florida, Orlando, FL, November 7, 2002.

 

(4) Professional, Scientific Conference Presentations:

[* Undergraduate students.]

Timerman, R.*, Chang Thao*, R. Welty, D. Wickramasinghe, and A.A. Heikal. “What kind of microviscosity does a molecule experience during its rotational and translational diffusions in crowded environments?” The 58th Biophysical Society Meeting, San Francisco, CA (February 15-19, 2014). Control Number: 14-A-3639-BPS, Poster Presentation.

Alfveby, J., R. Timerman*, J. Bartusek*, D. Wickramasinghe, H. Israelson*, A.A. Heikal. “Two-photon fluorescence lifetime imaging of natural coenzymes in living cells as a function of oxidative stress”, The 58th Biophysical Society Meeting, San Francisco, CA (February 15-19, 2014). Control Number: 14-A-4025-BPS, Poster Presentation.

Sheets, E.D., R. Welty, A.A. Heikal. “Macromolecular crowding effects on multiscale diffusion of single molecules”, The 58th Biophysical Society Meeting, San Francisco, CA (February 15-19, 2014). Control Number: 14-A-4179-BPS, Poster Presentation.

Thao, C.*, R. Timerman*, and A.A. Heikal. “Diffusion and microviscosity in crowded environments” The McNair Scholars Program, University of Wisconsin-Superior, September 25, 2013.

Welty, R., J. Bentley, D. Wickramasinghe, and A.A. Heikal. Multiscale diffusion of single molecules in biomimetic crowding. Biophysical Society Annual Meeting, Philadelphia, PA, February 2-6, 2013.

Fransen*, T., M.S. Velasquez*, K. Hewawasam, D. Wickramasinghe, R. Welty, and A.A. Heikal. Experimental and simulation analysis of NADH-enzymes binding in a crowded environment. Biophysical Society Annual Meeting, Philadelphia, PA, February 2-6, 2013

Welty, R., J. Bentley*, D. Wickramasinghe, and A.A. Heikal. Multiscale diffusion of single molecules in biomimetic crowding. The 245th ACS Annual Meeting, Division of Biological Chemistry, Session: Current Topics in Biological Chemistry, New Orleans, LA, April 7-11, 2013.

Fransen*, T., M.S. Velasquez*, K. Hewawasam, D. Wickramasinghe, R. Welty, and A.A. Heikal. Experimental and simulation analysis of NADH-enzymes binding in a crowded environment. The 245th ACS Annual Meeting, Division of Analytical Chemistry, Session: Advances in Analytical Spectroscopy, New Orleans, LA, April 7-11, 2013.

Welty, R., J. Bentley*, D. Wickramasinghe, and A.A. Heikal. "Diffusion dynamics of single molecules confined in biomimetic crowded environment", Joint ACS Midwest /Great Lakes Regional Meeting, St. Louis, MO, October 19 – 22, 2011

Wickramasinghe, D., J.Bartusek*, and A.A. Heikal.“Single molecule studies of Bodipy-FTY720 immune suppressor in biomembranes.” The 17th International Workshop on Single Molecule Spectroscopy and Ultra Sensitive Analysis in the Life Sciences, Berlin, Germany, September 7 – 9, 2011.

Welty, R., J. Bentley*, D. Wickramasinghe, and A.A. Heikal.“Multiscale dynamics of single molecules in biomimetic crowding,” The 12th Conference on Methods and Applications of Fluorescence (MAF-12), Strasbourg, France, September 11 – 14, 2011.

Bartusek, J.*, and A.A. Heikal. Partitioning, stoichiometry, and diffusion dynamics of FTY720 (an immune modulator) in Triton X-100 micelles. The Minnesota Academy of Science Annual Meeting, Winchell Undergraduate Research Symposium, Northfield, MN, April 16, 2011. “Best of Session Poster Presentation” Award.

Wickramasinghe, D., R. Bittman, and A.A. Heikal.Partitioning and single-molecule diffusion dynamics of Bodipy-FTY720 in biomembranes. The 55th Annual Biophysical Society Meeting, Baltimore, MD, March 5 – 9, 2011.

Porter*, J.J., R. Reed, T. Fisher, and A.A. Heikal. Molecular crowding effects on multiscale diffusion as a function of the hydrodynamic volume of both the solute and solvent molecules. The 53rd Biophysical Society Meeting, Boston, MA, February 28 – March 4, 2009.

Ariola, F.S., and A.A. Heikal. Examining the role of lipid variations and proteins on membrane biophysics: synthetic versus natural membrane vesicles. The 53rd Biophysical Society Meeting, Boston, MA, February 28 – March 4, 2009.

Yu, Q., and A.A. Heikal.Noninvasive Approach for quantitative analysis of energy metabolism and mitochondrial anomalies in living cells. The 53rd Biophysical Society Meeting, Boston, MA, February 28 – March 4, 2009.

Sheets, E.D., and A.A. Heikal. Membrane nanodomains facilitate immunoreceptor signaling. Biomedical Engineering Society Annual Meeting, Pittsburgh, PA, October 7 – 10, 2009.

Yu, Q., A.T. Lutes, and A.A. Heikal. 2008. Cellular autofluorescence: towards quantitative biochemistry associated with cell pathology and respiratory state activities. Biomedical Engineering Society Annual Fall Meeting, St. Louis, MO, October 2 – 4.

Yu, Q., A. Lutes, and A.A. Heikal. 2008. Two-Photon autofluorescence dynamics for quantitative cell pathology and respiratory state activities. Frontiers in Optics (FiO)/Laser Science XXIV (LS) Conference, Rochester, NY, October 19 – 23.

Ariola, F.S. and A.A. Heikal. 2008. Membrane fluidity and lipid order in synthetic and natural membrane vesicles. Biomedical Engineering Society Annual Fall Meeting, St. Louis, MO, October 2 – 4.

Davey, A., K. Krise, E. Sheets, and A. Heikal. 2008. Comparative studies of physiological and non-physiological crosslinking of IgE receptor in RBL mast cells. Biomedical Engineering Society Annual Fall Meeting, St. Louis, MO, October 2–4.

Yu, Q., R. Walvick, and A.A. Heikal. 2008. Fluorescence-based biochemistry on intrinsic cofactors in human breast normal and cancerous cells. 52st Annual Biophysical Society Meeting (Session: Oxidative Phosphorylation), Long Beach, CA, February 2–6. 

Lutes, A.T., Q. Yu and A.A. Heikal. 2008. Fluorescence dynamics and concentration imaging of intrinsic flavin at the single cell level. 52st Annual Biophysical Society Meeting (Session: Oxidative Phosphorylation), Long Beach, CA, February 2–6.

Sheets, E.D., A.M. Davey, K.M. Krise, and A.A. Heikal. 2008. Imaging membrane dynamics participating in immunoreceptor signaling. 52st Annual Biophysical Society Meeting (Session: Motions of Cell Surface Molecules), Long Beach, CA, February 2–6.

Davey, A.M., K.M. Krise, A.A. Heikal, and E.D. Sheets. 2008. Dynamics imaging of membrane nanostructural changes associated with antigen-mediated IgE receptor signaling in mast cells. 52st Annual Biophysical Society Meeting (Session: Lipids and Signaling on Membrane Surfaces), Long Beach, CA, February 2–6.  

Davey A.M., K.M. Krise, A.A. Heikal, and E.D. Sheets. 2007. Molecular perspective of antigen-mediated IgE receptor signaling in mast cells.  47th Annual American Society for Cell Biology Meeting, Washington, DC, December 1–5.

Ariola, A.S., M. Kyoung., E.D. Sheets, M. Edidin, and A.A. Heikal. 2007. Lateral heterogeneity and diffusion patterns of major histocompatibility complex (MHC) class I proteins in membrane blebs. 47th Annual American Society for Cell Biology Meeting, Washington, DC, December 1–5.

Heikal, A.A. 2007. Molecular dynamics perspective of cellular autofluorescence: from single-cell to neural network in brain slices. 47th Annual American Society for Cell Biology Meeting, Washington, DC, December 1–5.

Yu, Q., M. Proia, and A.A. Heikal. 2007. Integrated Biophotonics Approach for Quantitative, Multiscale Biophysics of Biological Systems. 47th Annual American Society for Cell Biology Meeting, Washington, DC, December 1–5.

Yu, Q., M. Proia, and A.A. Heikal. 2007. Integrated biophotonics approach for quantitative and multiscale analysis of biological systems. Biomedical Engineering Society Annual Fall Meeting, Los Angeles, CA, Sept. 26–30.

Heikal, A.A. 2007. Molecular dynamics perspective of cellular autofluorescence: From a single cell to brain slices. Biomedical Engineering Society Annual Fall Meeting, Los Angeles, CA, Sept. 26–30.

Heikal, A.A. 2007. Fluorescence dynamics imaging of IgE receptor signaling in RBL mast cells. Biomedical Engineering Society Annual Fall Meeting, Los Angeles, CA, Sept. 26–30.

Ariola, F.S. and A.A. Heikal. 2007. Integrative biomedical imaging of lipid analogue interactions in engineered lipid bilayers. Biomedical Engineering Society Annual Fall Meeting, Los Angeles, CA, Sept. 26–30.

Proia, M.A., S.R. Arumugam, S.J. Benkovic, and A.A. Heikal. 2007. Single-molecule studies of forked DNA binding with helicase loading protein (gp59). American Chemical Society 234th National Meeting, Boston, MA, August 19–23.

Ariola, F.S., D.J. Mudaliar, R.P. Walvick, and A.A. Heikal.  2007. Ultrafast dynamics imaging of lipid analogue interactions in giant unilamellar vesicles.  American Chemical Society 234th National Meeting, Boston, MA, August 19–23.

Shi, K., A.A. Heikal, and Z. Liu. 2007. Single-shot two-photon action cross section measurement. CLEO' 07, Baltimore, MD, May 6 – 1.

Davey, A.M., E.D. Sheets, and A.A. Heikal. 2007. Correlating membrane nanostructure with IgE receptor crosslinking in mast cells:  An ultrafast dynamics approach. 51st Annual Biophysical Society Meeting, Baltimore, MD, March 3–7.

Heetderks, J.J., F.S. Ariola, P.S. Weiss, and A.A. Heikal. 2007. Quantitative analysis of lipid-marker and domain-domain interactions using ultrafast spectroscopy of carbocyanine dyes in binary-phase giant unilamellar vesicles.  51st Annual Biophysical Society Meeting, Baltimore, MD, March 3–7.

Ariola, F.S., D.J. Mudaliar, R.P. Walvick, and A.A. Heikal. 2007. Examining lipid-marker interactions in model membranes using ultrafast dynamics imaging. 51st Annual Biophysical Society Meeting, 51st Annual Biophysical Society Meeting, Baltimore, MD, March 3–7.

Yu, Q., R.P. Walvick, F.S. Ariola, Y. Liu, and A.A. Heikal. 2007. Molecular dynamics imaging of intrinsic NAD(P)H in breast normal and cancer cells at the single cell level. 51st Biophysics Society Meeting, Baltimore, MD, March 3–7.

Yu, Q., M. Proia, and A.A. Heikal. 2007.  Integrated biophotonics approach for non-invasive and quantitative analysis of multiscale biological systems. 51st Biophysics Society Meeting, Baltimore, MD, March 3–7.

Davey, A.M., E.D. Sheets, and A.A. Heikal.  2006.  Correlating membrane nanostructure with IgE receptor crosslinking in mast cells:  an ultrafast dynamics approach.  The American Society for Cell Biology, San Diego, December 9–13.

Yu, Q., and A.A. Heikal. 2006. Non-invasive dynamics assay of quantitative analysis of cellular mitochondria. Biomedical Engineering Society Annual Fall Meeting, Chicago, IL, October 11–14.

Yu, Q., V. Aravamuthan, F. Ariola, Y. Liu, and A.A. Heikal. 2006. Dynamics imaging of intrinsic NAD(P)H in breast cancer cells as a biosensor of cellular physiology. Biomedical Engineering Society Annual Fall Meeting, Chicago, IL, October 11–14.

Muthukrishnan, G., Y. Liu, A.A. Heikal, and W.O. Hancock. 2006. Single molecule fluorescence measurements uncover differences between Kinesin-1 and Kinesin-2 motors. Biomedical Engineering Society Annual Fall Meeting, Chicago, IL, October 11–14.

Heikal, A.A. 2006. Short-range dynamics imaging of lipid phases in synthetic and natural biomembranes. The American Chemical Society Meeting, San Francisco, CA, September 9–14; Invited Talk.

Sheets, E.D., A.M. Davey, R.P. Walvick, and A.A. Heikal. 2006. Visualizing and probing molecular order and dynamics of lipid rafts in mast cells. The American Chemical Society Meeting, San Francisco, CA, September 9–14.

Sheets, E.D., A.M. Davey, and A.A. Heikal. 2006. Visualizing and probing molecular nanostructure in living mast cells.  38th Middle Atlantic Regional ACS Meeting, The Analytical Chemistry of Cellular Interactions with Biomaterial Surfaces, Hershey, PA.  June 4–7.

Sheets, E.D., A.M. Davey, and A.A. Heikal. 2006. Correlating membrane nanostructure and signaling activity in living mast cells using dynamics imaging.  FASEB Summer Research Conference, Molecular Biophysics of Cellular Membranes, Saxtons River, VT.  July 1-6. 

Davey, A.M., Y. Liu, A.A. Heikal, and E.D. Sheets. 2006. Visualizing and probing the order and molecular dynamics of lipid rafts in mast cells.  Frontiers in Live Cell Imaging, Bethesda, MD.  April 19–21.

Zhang, Y. D.J. Mudaliar, A.A. Heikal, and W.O. Hancock. 2006. Comparison of Kinesin-2 and Kinesin-1 processivity and microtubule affinity. Biophysical Society Annual Meeting, Salt Lake City, UT, February 18–22.

Davey, A.M., K. Vats, A.A. Heikal, and E.D. Sheets. 2006. Visualizing and Probing the Order and Molecular Dynamics of Lipid Rafts. Biophysical Society Annual Meeting, Salt Lake City, UT, February 18–22.

Mudaliar, D.J., R.P. Walvick, A.A. Heikal. 2005. Short-range dynamics of lipid domains in model biomembranes. The American Society for Cell Biology Conference, San Francisco, CA, December 10–14.

Walvick, R.P. and A.A. Heikal.  2005. Quantitative analysis of energy metabolism pathways in breast cancer cells using native NAD(P)H. The American Society for Cell Biology Conference, San Francisco, CA, December 10–14.

Walvick, R.P., D.J. Mudaliar, and A.A. Heikal. 2005. Accurate estimate of biomolecular concentration in living cells as high spatial resolution. The International Biophysics Congress, Montpellier, France, August 26 – September 1. 

Mudaliar, D.J., R.P. Walvick, and A.A. Heikal.  2005.  Lipid-phase specificity of rotational diffusion and molecular orientation in biomembranes.  The International Biophysics Congress, Montpellier, France, August 26 – September 1.

Kim*, H.-R., R.P. Walvick, W.M. Rivera*, Y. Liu, and A.A. Heikal.  2005.  Rotational complexity of enzyme-cofactor binding under equilibrium conditions.  Femtochemistry VII Conference, Washington, DC, July 17–22. 

Walvick, R.P. and A.A. Heikal.  2005.  Quantitative analysis of biomolecular concentration in living cells with high spatial resolution.  The Institute of Electrical and Electronic Engineers Conference, Hoboken, NJ, April 1–5.

Mudaliar, D.J., R.P. Walvick, and A.A. Heikal.  2005.  Lipid-order fluctuation in model membranes at high spatio-temporal resolution.  The 49th-Biophysical Society Meeting, Long Beach, CA, February 12–16.

Zhang, Y., D.J. Mudaliar, A.A. Heikal, W.O. Hancock. 2005. Microtubule-binding affinity and structural studies of EGFP-tagged KIF3A/B motors. The 49th-Biophysical Society Meeting, Long Beach, CA, February 12-16.

Rivera*, W.M., H.-R. Kim*, and A.A. Heikal. 2004.  Endogenous flavin and NADH fluorescence as a probe for energy metabolism. Annual Biomedical Research Conference for Minority Students, Dallas, TX, November 10–13.

Heikal, A.A., J. Korlach, D.W. Baird, G.R. Hoffmann, K. Gee, R.A. Cerione, W.W. Webb.  2004.  Structural aspects of g-phosphate tagged GTP analogs for G proteins studies.  50th Anniversary Meeting of the Gordon Conference on Isotopes in Biological and Chemical Sciences, Ventura, CA, February 15–20.

Heikal, A.A., H. Vishwasrao, K.A. Kasischke, and W.W. Webb. 2003. Conformational changes of native NADH associated with neural energy metabolism in hippocampal slices. 43rd Meeting of the American Society for Cell Biology, San Francisco, CA, Dec 13–17. 

Sheets, E.D., S.T. Hess, A. Wagenknecht-Wiesner, and A.A. Heikal.  2003.  Quantitative analysis of the rotational flexibility of Lyn-B protein in living RBL mast cells. 43rd Meeting of the American Society for Cell Biology, San Francisco, CA, Dec 13–17.

Korlach, J., B. Baird, A.A. Heikal, K.R. Gee, G.R. Hoffman, and W.W. Webb. 2003. Spontaneous nucleotide exchange of fluorescently labeled g-phosphate linked GTP analogs in low molecular weight GTPases. 43rd Meeting of the American Society for Cell Biology, San Francisco, CA, Dec 13–17.

Vishwasrao, H.D., A.A. Heikal, K.A. Kasischke, and W.W. Webb. 2003. Two-photon time and wavelength resolved spectroscopy probes conformational changes of intrinsic NADH associated with neural energy metabolism. 33rd Annual Meeting of Society for Neuroscience, New Orleans, LA, November 8–12.

Kasischke, K.A., H.D. Vishwasrao, P.J. Fisher, A.A. Heikal, W.R. Zipfel, and W.W. Webb. 2003.  Two-photon NADH imaging resolves functions of astrocytes and neurons in metabolic recovery during focal neural activity. 33rd Annual Meeting of Society for Neuroscience, New Orleans, LA, November 8–12.

Heikal, A.A., H. Vishwasrao, K. Kasischke, and W.W.Webb. 2003. Metabolic activity effects on the excited state dynamics of native NADH in brain slices. Frontiers in Optics and Laser Sciences XIX, Tucson, AZ, October 5–9.

Heikal, A.A. and W.W. Webb.  2002. Multiphoton fluorescence microscopy in biology. SPIE-The International Society for Optical Engineering, Nonlinear Spectroscopy, Seattle, WA, USA, July 08.

Kasischke, K., H. Vishwasrao, A.A. Heikal, and W.W. Webb. 2002. Two-photon redox fluorimetry: a new functional imaging technique for visualizing of energy metabolism in brain tissue. The Biophysical Society Meeting, San Francisco, CA, February 23–27.  

Vishwasrao, H., A.A. Heikal, K. Kasischke, and W.W. Webb. 2002. Probing cellular redox state with time resolved multiphoton spectroscopy. The Biophysical Society Meeting, San Francisco, CA, February 23–27.

Hess, S.T., A.A. Heikal, and W.W. Webb. 2002. Effects of mutations on photophysics of intrinsically fluorescent proteins. The Biophysical Society Meeting, San Francisco, CA, February 23–27.

Heikal, A.A., S. Huang, M. Halik, S.R. Marder, W. Wenseleers, J.W. Perry, and W.W. Webb. 2002. Newly designed two-photon fluorescence markers for biological applications. The Biophysical Society Meeting, San Francisco, CA, February 23–27.

Heikal, A.A., S.T. Hess, E.D. Sheets, and W.W. Webb. 2001. Color carnival by marine life: understanding the molecular dynamics and spectroscopy of intrinsically fluorescent proteins. Vth Femtochemistry Conference, Toledo, Spain, September 2.

Heikal, A.A., S. Huang, M. Halik, S.R. Marder, W. Wenseleers, J.W. Perry, and W.W. Webb. 2001. Fluorescence spectroscopy, dynamics and imaging of designed two-photon fluorescent markers in Triton X-100 micelles and RBLcells. Opto Northeast and Imaging 2001: Biomedical Optics and Imaging Session, Rochester, NY, April 10.

Hess, S.T., A.A. Heikal, and W.W. Webb. 2001. Advantageous molecular photophysical fluorescence properties of DsRed and Citrine. The Biophysical Society Meeting, Boston, MA, February 17–21.

Heikal, A.A., J. Korlach, and W.W. Webb. 2001. Time-resolved fluorescence and anisotropy of free and DNA-bound fluorescently labeled nucleotides. The Biophysical Society Meeting, Boston, MA, February 17–21.

Hess, S.T., A.A. Heikal, and W.W. Webb. 2000. Comparative single molecule photophysics of green fluorescent proteins: mutation effects. The 6th International Workshop on Single Molecule Detection and Ultrasensitive Analysis of Life Sciences, Berlin-Adlershof, Germany, September 27.

Heikal, A.A., S.T. Hess, and W.W. Webb. 2000. Comparative ultrafast molecular dynamics of selected green fluorescent proteins. American Chemical Society, 220th PHYS-553, Washington DC, August 21-25.

Heikal, A.A., J. Korlach, and W.W. Webb. 2000. Time-resolved fluorescence and anisotropy of free and DNA-bound fluorescently labeled nucleotides. American Chemical Society, 220th PHYS-553, Washington DC, August 21–25.

Heikal, A.A., S.T. Hess, P. Schwille, and W.W. Webb. 2000. Ultrafast molecular dynamics of selected green fluorescent protein mutants. The Biophysical Society Meeting. New Orleans, LA, February 12–16.

Hess, S.T., A.A. Heikal, and W. W. Webb. 2000. Reversible light-induced proton transfer reaction in ecliptic green fluorescent protein. The Biophysical Society Meeting, New Orleans, LA, February 12–16.

Huang, S., A.A. Heikal, and W.W. Webb. 2000. Multiphoton fluorescence spectroscopy of LipDH, FAD, and NAD(P)H. The Biophysical Society Meeting, New Orleans, LA, February 12–16.

Heikal, A.A., P. Schwille, S.T. Hess, and W.W. Webb. 1999.  pH- and excitation wavelength-dependence of one- and two-photon fluorescence spectroscopy and dynamics of enhanced green fluorescent protein (EGFP).  2nd International Symposium on GFP, San Diego, CA, May 22.