Dynamics Imaging of Biomolecular Assemblies

 

Research

A. Energy Metabolism and Mitochondria

  1. Heikal, A.A. Intracellular coenzymes as natural biomarkers for metabolic activities and mitochondrial anomalies. Biomarkers in Medicine, 4(2): 241-263 (2010); Invited Review.
  2. Yu, Q. and A.A. Heikal. Two-photon autofluorescence dynamics imaging reveals sensitivity of intracellular NADH concentration and conformation to cell physiology at the single-cell level. Journal of Photochemistry and Photobiology (B): Biology, 95: 46–57 (2009); Press Release.
  3. Vishwasrao, H.D., A.A. Heikal, K.A. Kasischke, and W.W. Webb. Conformational dependence of intracellular NADH on metabolic state revealed by associated fluorescence anisotropy. J. Biol. Chem., 280:25119-25126 (2005); Press Release, Issue Cover and Paper of the Week.
  4. Huang, S., A.A. Heikal, and W.W. Webb. Two-photon fluorescence spectroscopy and microscopy on NAD(P)H and flavoprotein, Biophys. J., 82:2811-2825 (2002).

Mitochondrial Function (Heikal, Biomarkers in Medicine, 2010)

B. Biomimetic Membranes

  1. Ariola, F.S., Z. Li, C. Cornejo, R. Bittman, and A.A. Heikal. Membrane fluidity and lipid order in ternary giant unilamellar vesicles using a new Bodipy-cholesterol derivative. Biophysical Journal, April Issue, 96(7), 2696–2708 (2009).
  2. Ariola, F.S., D.J. Mudaliar, R.P. Walvick, and A.A. Heikal. Dynamics imaging of lipid phases and lipid-marker interactions in model biomembranes. Phys. Chem. Chem. Phys., 8:4517-4529 (2006).

Phase Separation in GUVs Labeled with Bodipy-Cholesterol and DiI-C12 (Ariola et al. Biophys. J. 2009)

C. Immunoglobulin E Receptor Signaling

  1. Davey, A.M., K. Krise. E.D. Sheets, and A.A. Heikal. Molecular perspective of antigen-mediated mast cell signaling. J. Biol. Chem., 283:7117-7127 (2008); Press Release.
  2. Davey, A.M., R.P. Walvick, Y. Liu, A.A. Heikal, and E.D. Sheets. Membrane order and molecular dynamics associated with IgE receptor crosslinking in mast cells. Biophys. J., 92:343-355 (2007).

IgE Receptor Signaling Pathway (Davey et al, Biophys. J., 2007)

D. Intrinsically Fluorescent Proteins

  1. Liu, Y., H.-R. Kim, and A.A. Heikal. Structural basis of fluorescence fluctuation dynamics of green fluorescent proteins in acidic environments. J. Phys. Chem. B, 110:24138-24146 (2006).
  2. Hess, S.T.,E.D. Sheets, A. Wagenknecht-Wiesner, and A.A. Heikal. Quantitative analysis of the fluorescence properties of intrinsically fluorescent proteins in living cells. Biophys. J., 85:2566-2580 (2003). 
  3. Heikal, A.A., S.T. Hess, G.S. Baird, R.Y. Tsien, and W.W. Webb. Molecular spectroscopy and dynamics of intrinsically fluorescent proteins: coral red (dsRed) and yellow (Citrine). Proc. Natl. Acad. Sci. USA, 97:11996-12001 (2000).

E. Micro-spectroscopy & Technique Development

  1. Yu, Q., M. Proia, and A.A. Heikal. Integrated biophotonics approach for noninvasive and multiscale studies of biomolecular and cellular biophysics. J. Biomed. Optics; July/August Issue, 13(4):0413151-14, (2008).