B
S
L |
Agents |
Practices |
Safety Equipment (Primary Barriers)
|
Facilities
(Secondary
Barriers) |
| 1 |
Not
known to consistently cause disease in healthy adults |
Standard
Microbiological Practices |
None
required |
Open
bench top sink required |
| 2 |
Associated
with human disease hazard: percutaneous injury, ingestion,
mucous membrane exposure
|
BSL-1
practice plus:
Limited access
Biohazard warning signs
"Sharps" precautions
Biosafety manual defining
any needed waste decontamination or medical surveillance policies
|
Primary
barriers: Class I or II BSCs, or other physical containment
devices used for all manipulations of agents that cause splashes or
aerosols of infectious materials; PPEs: laboratory coats; gloves; face
protection as needed
|
BSL-1
plus: Autoclave available
|
| 3 |
Indigenous
or exotic agents with potential for aerosol transmission; disease may
have serious or lethal consequences |
BSL-2
practice plus:
Controlled access
Decontamination of all waste
Decontamination of lab
clothing before laundering
Baseline serum
|
Primary
barriers: Class I or II BCSs or other physical containment
devices used for all open manipulations of agents; PPEs: protective lab
clothing; gloves; respiratory protection as needed
|
BSL-2 plus:
Physical separation from
access corridors
Self-closing, double-door
access
Exhausted air not
recirculated
Negative airflow into
laboratory
|
| 4 |
Dangerous/exotic
agents which pose high risk of life-threatening disease,
aerosol-transmitted lab infections; or related agents with unknown risk
of transmission |
BSL-3
practices plus:
Clothing change before
entering
Shower on exit
All material decontaminated
on exit from facility
|
Primary
barriers: All procedures conducted in Class III BSCs or Class I
or II BSCs in combination with full-body, air-supplied,
positive pressure personnel suit
|
BSL-3 plus:
Separate building or
isolated zone
Dedicated supply and
exhaust, vacuum, and decon systems
Other requirements outlined
in the text
|
