Intended For On-Site Autoclaving
All infectious/ objectionable (other than sharps and research animal
waste) intended for on-site decontamination by autoclaving will be
placed immediately into approved (see general requirement above),
prominently labeled, leak-burst-tear, and puncture proof containers at
the point of generation.
Waste Disposal Procedure
- Containers will be lined with
a prominently, preferably clear, autoclavable plastic bag.
- When bag is 3/4 full, it will be sealed and
taken immediately to the autoclave room for decontamination.
- Infectious waste bags will not be handled in
such a manner,
which might cause them to break open, spill, or contaminate the
- Waste will be autoclaved for at least
60-minute cycles at 121C
- Autoclaved waste bags will be drained from
any residual liquid in the autoclave room sink.
- Drained autoclaved bags will then be placed
in the provided normal solid waste container located in the autoclave
room, and disposed of as normal waste.
Infectious Waste Disposal Procedure
- All infectious
wastes that cannot be decontaminated or autoclaved
will be placed immediately in red infectious waste bags. When the bag
is 3/4 full, or at the end of experiment or procedure, depending on the
infectious agent. The bag will be sealed, placed in a disposal
container and stored in freezers, refrigerators, or cold rooms until
shipment to outside disposal facility.
- All UMD generated infectious waste that is
on site including sharps will be shipped to the University of
Minnesota, Minneapolis campus, and added to their infectious waste
stream destined for terminal destruction (autoclaving and grinding or
incineration). The waste will be shipped in approved leak, burst, and
puncture proof, prominently labeled HDPE drums. The drums will be
sealed tightly and transported by the University of Minnesota hazardous
infectious waste such as blood and regulated body fluids
will be discarded/flushed directly down the sanitary sewer with copious
amounts of water at the point of generation.
- If liquid infectious wastes are in leak proof
(e.g. blood transfusion bags) that cannot be emptied and flushed down
the sanitary sewer, the container will be placed directly into an
infectious waste collection bag regardless of its infectious nature,
and sent for autoclaving or disposal as infectious non-autoclavable
All used disposable sharps will be
placed directly into approved prominently labeled, leak and
puncture-resistant red disposable sharps containers.
- Sharps receptacles will be placed in a
convenient to the
activity generating the waste (e.g. laboratory workbench, patient room,
or surgical room) to ensure proper segregation and packaging of the
waste, and to minimize risk of injury to laboratory and housekeeping
- Used disposable needles and syringes will be
placed, intact, directly into waste receptacle without recapping.
- Sharps containers will not be overfilled, but
removed and replaced when 3/4 full.
- Sharps will not be forced into sharps
receptacle, but should fit easily into the container being used.
- Sharps will not be left unattended where they
- Filled sharps containers will be closed
tightly and sealed by
laboratory personnel generating the waste, to avoid spillage during
transportation by laboratory and housekeeping personnel.
- Sharps will not be disposed of in broken
glass containers or normal trash.
- Sharps receptacles use will be restricted to
the collection and management of sharps. No other uses will be allowed.
- Sharps containers for needles, syringes,
blades, etc., will never be placed in the normal waste container even
- All laboratories involved with the use
agents must decontaminate all cultures, stocks and materials used in
the manipulation of infectious agents before disposal into the normal
waste stream or general refuse.
- Infected animal carcasses and body parts will
not be decontaminated on-site but shipped for off-site incineration.
- Subsequent to decontamination, all autoclaved
waste will be handled as normal waste and discarded in the normal waste
Sharps contaminated with
will be segregated from other sharps and managed separately.
Radioactive sharps will be packaged according to above outline,
and University of Minnesota regulations regarding the management of
In general, radioactive sharps will be packaged into leak and
puncture-resistant sharps containers, as outlined above.
Radioactive sharps containers will be prominently labeled with
the word "Sharps" and bearing the radioactive symbol as well as the
isotope name and activity. Consult the Radiation Protection
Manual for more information on radioactive waste management procedure,
or call Environmental Health and Safety Office at 726-7273.
Extremely Hazardous Substances:
Sharps contaminated with toxic or
extremely toxic or bio-accumulative
chemical substances such as PCB's, mercury, heavy metals, etc., will be
segregated from other sharps at the generation point and managed as
hazardous chemical waste sharps according to their hazard category. The
procedure outlined above for the management of sharps will hold.
Containers of sharps contaminated with hazardous chemicals will be
labeled as sharps and bear the name of the hazardous contaminant.
As is the case for the disposal of hazardous chemical waste,
manifesting for the disposal of sharps contaminated with chemical waste
is required. For more information on the management and disposal of
hazardous chemical waste, consult the University of Minnesota Hazardous
Chemical Waste Management Guidebook 5th Edition, or call the
Environmental Health and Safety Office at 726-7273.
Glass Disposal Procedure
non-contaminated broken glass items
will be placed in
containers such as a designated cardboard box. Containers will be
labeled "Broken Glass" so that custodial workers know they are handling
- Glassware that has
been contaminated with blood
infectious agents must be disinfected before disposal in the broken
glass container. Otherwise, it will be disposed in appropriate sharps
container as infectious waste.
- Broken glass
contaminated with hazardous
material will be disposed of as hazardous waste.
thermometers are considered hazardous
waste and should be disposed of as such.
- Broken glass contaminated with radioactive
materials will be disposed of as radioactive waste.
- Discarded non-broken glass that is not
must be residue-free and then disposed of in a broken glass container.
- Non-broken glass that is intended for recycling
will be decontaminated, rinsed from any remaining residue first, and
Waste Disposal Procedure
The University of Minnesota-Duluth has
received approval form the WLLSD
to dispose of its non-infected animal waste (animal carcasses, body
parts), including preserved animals in their Rice Lake Industrial and
Solid Waste Disposal Facility.
This agreement precludes infected, radioactive research animal waste,
and research animal waste contaminated with extremely toxic chemicals.
General Animal Waste
- All animal carcasses and body parts will
immediately in impervious (heavy duty), prominently labeled, leak and
tear-resistant waste bags depending on animal waste classification (red
infectious waste bags for infectious animal waste) at the point of
- All sharp objects such as needles, capillary
tubes, and razor
blades will be removed from animals before placing them in collection
- Bags must be labeled according to hazard class
infectious, infectious, radioactive, or hazardous waste) and stored
- Bags will be sealed and placed in a disposal
stored in appropriate/ approved freezers, refrigerators, or cold rooms
to prevent putrefaction, and minimize odors while awaiting shipment to
an outside facility or ultimate disposal.
- Body fluid (urine) waste from large infected
observation will be flushed into the sanitary sewer with copious
amounts of water.
- Bedding from animals intentionally infected
agents known to be infectious to humans will be disposed of in an
infectious waste bag, and decontaminated by autoclaving.
- Bedding from non-infected animals is not
considered infectious and will be collected in normal waste bags and
disposed of in the regular/ normal waste or refuse.
Special Animal Waste
- Preserved and
non-infectious animal waste
from the biology department, medical school and animal
services will be packaged, stored and disposed of according to the
outline and agreement with WLLSD and delivered by UMD trained personnel
to the Rice Lake Industrial and Solid Waste Disposal Facility.
- All research
animal waste disposed of at the
Industrial and Solid Waste Disposal Facility will satisfy the
following: (1) not contained and heavy metals, (2) not contain any zero
discharge chemicals (see appendix 1 for list of zero discharge
chemicals), (3) not contain any toxic or extremely toxic chemicals at
concentrations of oral rat LD50 x dilution rate < 500ppm, (4) not
have been in contact with any radioactive materials.
- All infectious animal wastes (carcasses
body parts) will
be packaged according to the above guidelines in prominently labeled
tear and leak-resistant red biohazard waste bags bearing the words
"infectious waste" and/or the biohazard symbol. Sealed immediately and
placed in an approved freezer until shipment as infectious waste. The
UMD Environmental Health and Safety Office must be contacted at
726-7273 to arrange for disposal.
Radioactive animal waste and animal waste
contaminated with hazardous
or highly toxic chemicals are not discussed here. For information on
radioactive animal waste disposal procedure, consult the University of
Minnesota Radiation Protection Manual. For more information on the
disposal of animal waste contaminated with toxic chemicals consult the
University of Minnesota Chemical Waste Management Guidebook 5th edition
of call the UMD Environmental Health and Safety Office at (218)
pathological waste must be managed and disposed of according to
sanitary standards established by state, federal laws and regulations
governing the use and disposal of such waste.
- All human
pathological wastes generated outside of the Anatomy and
Cell Biology Department, that has been used in research and/or
scientific investigations will be placed into impervious, tear and
burst resistant plastic bags, which will be placed in rigid containers
that are labeled "Pathological" or "Infectious Waste". Containers will
be then stored in appropriate freezers or coolers at the generating
department. Arrangements must be made with Environmental Health and
Safety Office for disposal of such waste.
- All human
pathological waste generated
outside the Department
of Anatomy will be transported to the crematory in 1-156 Jackson Hall
- Staff at the
crematory will be responsible
for placing the
pathological waste bags into the hearth of the crematory and completing
cremation of this waste.
- Pathological materials from the Body
Program of the
Medical School will be handled by the Anatomy Department. When studies
are completed, pathological material will be placed in sealed
containers according to the above outline, and then shipped to the Twin
Cities Campus to be cremation.