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ILSC Monthly Seminar: April 19, 2012


January: Michael Taylor
February: Marna Yandeau-Nelson
March: Paul Lockman
April: Martin Gruebele


January: David D. Thomas
February: Elizabeth Austin-Minor
March: Juliette Lecomte
April: David Millar
May: Lukas Tamm
September: David Millar
September: Dongping Zhong
October: Jonathan V. Rocheleau
November: Yevgenya Grinblat
December: Michael Graner


January: Clay Carter
February (a): Anne Kenworthy
February (b): Jennifer Liang
March: Joe Johnson
April: Marco Ciufolini
May: Gary Pielak
October: Matthew Andrews
December: Andrew Skildum


November: Rui Wang
October: Marshall Hampton

Nucleic Acid–Protein Interactions at the Single-Molecule Level

Speaker: Dr. David Millar;
Department of Molecular Biology, The Scripps Research Institute, La Jolla, California

Time/Place: Thursday, April 19th at 3:00 pm, 150 SMed

Abstract: Single-molecule fluorescence spectroscopy is emerging as a powerful tool for detailed biophysical analyses of nucleic acid–protein interactions. Measurements at the single-molecule level can readily resolve and quantify multiple binding modes in complex interaction systems. Moreover, kinetic information on individual binding and dissociation steps or conformational changes can be obtained under conditions of thermodynamic equilibrium, without the need to synchronize a population of molecules. To illustrate these capabilities, I will describe two systems currently under study in my laboratory. (1) The HIV-1 protein Rev interacts with the Rev response element to mediate nuclear export of viral mRNAs. (2) DNA polymerases undergo large conformational changes as they select cognate dNTP substrates during DNA replication.

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