Date

#

Topics

Lab Manual Chapters

1/20

- - - no lab - - -

1/25

1

Introduction to Developmental Biology Lab; lab safety training (videos); preparation of embryological tools; microscope set-up.

Spermatogenesis & oogenesis (microscope slides)
Insect oogenesis: dissection of diff. ovariole types, experiment simulated yolk incorporation 

Chapters 1 & 2

Chapter 5
Handout

2/1

- - - no lab - - -

2/3

3

Sea urchin development (microscope slides, video, online materials)

Experiments with sea urchins (2 lab reports):
interfering with fertilization, induction of parthenogenesis, development from single blastomeres, effects of UV and environmental contaminants on development

2/8

4

2/17

7

2/22

8

Insect embryogenesis & postembryonic development:
induction of oviposition in flies; video Drosophila development
fly embryogenesis, larval imaginal discs;
hormonal control of pupation and metamorphosis; Drosophila salivary gland chromosomes

Chapter 8 & Handouts

2/24

9

3/1

10

Regeneration & pattern formation experiments with Planaria & Lumbriculus (lab report)

Fish development: video zebra fish development

Chapter 13 & handouts

Handouts

3/3

11

3/8

12

Early fish development: the Japanese Medaka

Fish experiments (lab report): sensitivity of early life stages of the Japanese Medaka to ethanol, retinoic acid, environmental contaminants

Regeneration experiments: continue observations & final evaluation

Handouts

Chapter 13 & handouts

3/10

13

3/15

14

3/17

15

3/21-25

3/29

16

Early development of amphibians: preserved developmental stages of frog, microscope slides, video;

Induction of ovulation and mating in Xenopus, normal development
Experiments with frog embryo (lab report): effects of lithium ions and trypan blue; set up exeriments with tadpoles;

3/31

17

4/5

18

4/7

19

4/12

20

Early development of the chick:
Video chick development; Microscope slides of chick embryo whole mounts & microscopic sections: 12 - 72h;

Preparation of 48 h - 72 h chick embryo whole mounts: dissection & fixation;

Experiments with chick embryo: (lab report)
Chorioallantoic membrane grafting with chick embryo limb buds;
Neural tube surgery with 72h embryo
In vitro culture and heart duplication with 24 h chick embryo;

Chapters 9-11; handouts

4/14

21

4/19

22

4/21

23

4/26

24

Hormonal regulation of amphibian metamorphosis:

Dissection of thyroid glands; experiments with Xenopus tadpoles

4/28

25

5/3

26

5/5

27