Chem 4242, Spring 2005

Part 4. Molecular Absorption and Fluorescence Spectroscopy and Mass Spectroscopy.

Topic Lecture Notes and Reference Materials
Days
 Reading/Study Assignment (SHN = Skoog, Holler, Nieman, 5e)
4A. UV-Visible Molecular Absorption Spectroscopy Bief review of some of the basic principles and applications. Principles and instrumentation including the Beer-Lambert Law, absorbance of mixtures, instrumental and chemical deviations from Beer's Law, effects of noise on absorbance readings, and some typical single-beam, double-beam, and diode-array instrument schematics. Applications including the nature of absorbing species, direct quantitation methods, photometric titrations. 1-2

Ch 13. An Introduction to UV-Visible Molecular Absorption Spectrometry. You should be able to answer all of the questions at the end of this chapter.

Ch 14. Applications of UV-Visible Molecular Absorption Spectrometry. Sections A, B, C, D1-D2, E. Suggested exercises: #1,2,5,6,8-11.
4B. Molecular Fluorescence Spectroscopy

Molecular Luminescence Methods. These methods include fluorescence, phosphorescence, and chemiluminescence. The emphasis in this discussion is on fluorescence.

Part I. Molecular Processes in Photoluminescence.

Part II. Instrumentation and Applications of Fluorescence.
2

Ch 15, sections A-D.

Suggested exercises: #1-6, 9, 11. See corrrections to #6, 8, 9.
4C. Atomic Mass Spectrometry

Mass Spectrometry. Basic aspects of mass spectrometry and mass spectrometers. Ion sources, analyzers, and mass spectra.

Atomic mass spectrometry lecture notes (ppt)
2

Ch 11. Atomic mass spectrometry, sections A-C.

  • A. General features of atomic MS
  • B. Mass spectrometers (transducers; quadrupoles, time-of-flight, double-focusing analyzers)
  • C. ICP-MS. Instrumentation, mass spectra, interferences

Suggested exercises: Ch 11 #1-9.
4D. Molecular Mass Spectroscopy

Molecular Mass Spectroscopy I

Molecular Mass Spectroscopy II

  • Major features of molecular mass spectra
  • Inlets, sources, mass filters, detectors
  • Ionization methods, including electron impact (EI), chemical ionization (CI), electrospray ionization (ESI), MALDI, others.
  • Mass analzyers: Resolution, magnetic focusing, double focusing, quadrupole, time-of-flight, ion trap.
  • Ion cyclotron resonance and Fourier Transform MS..
  • Ion detectors: Ion multiplier, faraday cup.
  • Characterization of pure compounds and mixtures
    • Isotope ratios, fragmentation patterns
  • Hyphenated techniques
    • GC/MS, LC/MS, CE/MS
    • Multiple MS
3

Ch 20. Molecular mass spectrometry, all sections

Suggested exercises: #1-11. 13-16. See correction for #6.

Additional resources:

Other important areas of molecular spectroscopy that you have seen in other courses include infrared spectroscopy and nuclear magnetic resonance (NMR). Your textbook includes discussions of these and other areas of molecular spectroscopy.

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