Thirteen-lined Ground Squirrel 

(Spermophilus tridecemlineatus)

Heart Tissue
Digital Gene Expression Assay

EST Contigs

 

 

The BLASTn algorithm was used to identify Expressed Sequence Tags (ESTs) expressed in the heart of thirteen-lined ground squirrels. ESTs which showed multiple identification tags were aligned as described by Brauch et al. to create the contigs found in this library. Occasionally multiple contigs were created from the same gene-specific ESTs in cases where complete alignment could not be achieved. These identifications are noted with the contig number in parentheses.

 

 

Click on name identification to access contig sequence.

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Name Identification
Length
Actin alpha cardiac 1462
Actinin, alpha 2 (ACTN2) 2124
Acyl-Coenzyme A dehydrogenase, long chain 1229
ADP,ATP translocase (solute carrier family 25) 1385
ADP-ribosyltransferase 3 1479
Aldolase A, fructose bisphosphate 1449
ATP synthase H+ transporting, mitochondrial F0 complex 815
ATPase Ca++ transporting, cardiac, slow twitch 2(SERCA2a) (Contig 1) 1149
ATPase Ca++ transporting, cardiac, slow twitch 2(SERCA2a) (Contig 2) 1219
Atrical natriuretic peptide precursor A 860
Barrier to autointegration factor 1 763
Calcium/calmodulin dependent protein kinase II delta 929
Calmodulin2 phosphorylase kinase, delta 778
CDK2-associated protein 1 (CDK2AP1), p12(DOC-1) (Contig 1) 1477
CDK2-associated protein 1 (CDK2AP1), p12(DOC-1) (Contig 2) 878
COP9 constitutive photomorphogenic homolog subunit 8 1326
Creatine phosphokinase (M isoenzyme) (Contig 1) 1195
Creatine phosphokinase (M isoenzyme) (Contig 2) 917
Cystatin F (leukocystatin) 857
Cytochrome b 1243
Deubiquitinating enzyme (UNPH4) 895

Elongation factor 1 A2

1032
Elongation factor 1 A1 1308
Eukaryotic translation initiation factor 3, subunit 5 (Contig 1) 581
Eukaryotic translation initiation factor 3 subunit 5 (Contig 2) 581
Eukaryotic translation initiation factor 4 gamma (Contig 1) 931
Eukaryotic translation initiation factor 4 gamma (Contig 2) 1505
Eukaryotic translation initiation factor 5a (Contig 1) 660
Eukaryotic translation initiation factor 5a (Contig 2) 660
Ferritin, heavy polypeptide 1 1089
Four and a half LIM domain ll (limpet) 2126
Glyceraldehyde-3-phosphate dehydrogenase 1303
Heart pancreatic triacylglycerol lipase 2439
Heart type fatty acid binding protein 736
Heat shock 70kDa Protein 8 1542
Hemoglobin alpha chain 593
Heparin sulfate 6-O-sulfotransferase (Contig 1) 977
Heparin sulfate 6-O-sulfotransferase (Contig 2) 947
Hydroxysteroid dehydrogenase-like protein 1413
Isocitrate dehydrogenase 2 (NADP+) 1528
KIAA0295 protein 908
Lactate dehydrogenase B 1269
Long-chain acyl-CoA dehydrogenase 1189
Lymphotoxin-beta (LT-beta) 1097
Lysosomal-associated protein transmembrane 4 alpha 1010
MEGF5 924
MLC2, nonmuscle myosin light chain 2 790
Myoglobin 1094
Myosin heavy chain alpha cardiac 1495
Myosin heavy polypeptide 7, cardiac muscle 1660
Myosin, heavy polypeptide 6, cardiac muscle 700
Myosin heavy chain slow isoform 822
Myosin light chain2 cardiac ventricular 1127
Myosin light chain kinase (Contig 1) 1105
Myosin light chain kinase (Contig 2) 800
Myosin light polypeptide2, regulatory, cardiac, slow 1026
Myosin regulatory light chain ventricular isoform 909
NADH dehydrogenase 1411
NADH dehydrogenase (ubiquinone) Fe-S protein 1 1207
NADH dehydrogenase subunit 1 2223
NADH dehydrogenase subunit 4 725
NADH-ubiquinone oxireductase AGGG subunit 523
NADH-ubiquinone oxireductase B8 subunit 462
NADH-ubiquinone oxireductase subunit 2 1150
Natruiretic peptide precursor A 842
ncx1 gene (exon 12) (Contig 1) 1124
ncx1 gene (exon 12) (Contig 2) 854
NDRG family member 4 1035
Nicotinamide nucleotide transhydrogenase 2138
NRF1 protein (NRF1) 810
NS1 binding protein 835
Peptidylprolyl isomerase D (cyclophilin D) 661
Phosphofructokinase (Contig 1) 1116
Phosphofructokinase (Contig 2) 756
Phospholamban 1535
Phosphorylase B Kinase Beta Regulatory Chain 777
Phosphorylase kinase, beta 706
PDZ and LIM domain 1 (elfin) 814
Plakoglobin 788
Poly(A) binding protein 717
Polyubiquitin 488
Preoxiredoxin 1 (PRDX1) 889
Putative glialblastoma cell differentiation-related protein 640
Putative leucine rich repeat protein 611
Putative NFkB activating protein HNLF 1050
Pyruvate dehydrogenase kinase isoform 4 1490
RAB1B Ras oncogene family 707
rab11 binding protein 839
Short interspersed element (SINE-B1-dID-like element) 1376
SLIT3 (UNQ691) 646
Slow skeletal troponin C (TnC) 787
Skeletal muscle alpha-tropomyosin (htM-alpha) 1270
Smhs2 homolog 893
Sorbin and SH3 domain containing 1 (SORBS1) (Contig 1) 1667
Sorbin and SH3 domain containing 1 (SORBS1) (Contig 2) 1524
Stathmin 877
Succinate-CoA ligase, ADP-forming, beta subunit 1196
Succinate-CoA ligase, GDP-forming, alpha subunit 1247
Succinate dehydrogenase complex subunit A, flavoprotein 833
Titin (Contig 1) 2271
Titin (Contig 2) 835
Titin-cap (telethonin) 1003
Transcriptional coactivator tubedown-100 (TBDN100) 805
Translation repressor NAT1 891
Trifunctional enzyme beta subunit 305
Triosephosphate isomerase 1 847
Ubiquinol-cytochrome c reductase hinge protein 715
Ubiquitin-activating enzyme E1 835
Ubiquitin carboxy-terminal hydrolase 1 890
Unactive progresterone receptor 23kD 905
Voltage-dependant anion channel 2 1335
Woodchuck hepatitis virus DNA 932
Zinc finger protein 574 888
 

Contact Information:

Matthew T. Andrews

University of Minnesota Duluth

Department of Biology

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