MABs / Ideal characteristics
Characteristics of an ideal MAB
-
High degree of affinity and specificity
- Adequate recruitment of effector functions
- strong response to pure mouse antibodies is an advantage
- not always necessary
- e.g., antibodies that target TNFα (adalimumab, golimumab, infliximab) are thought to have benefits by preventing TNFα from binding to its receptor
- can be increased by coupling to cytotoxic agent, e.g. ibritumomab
- Long half-life
- pure mouse antibodies are cleared rapidly; purely human antibodies can be detected >6 months after administration
- Reduced SYSTEMIC immunogenicity
(esp. want decreased HAMA ---human anti-mouse antibody --- response)
- HAMA response is greatest for mouse > chimeric > humanised > human least
- i.e., it is predictable based on amount of mouse protein present, which is a function of the antibody design